Category Archives: Protocol Development

Amphibians, DNA Marker Development, Hellbender Salamander (Cryptobranchus alleganiensis), LA, Multi Species Panel Development, Protocol Development, Research, Sample Processing

Kimberly Terrell

Kimberly Terrell
Wildlife Biologist
Smithsonian Conservation Biology Institute
Tulane University
New Orleans, LA 70118
Phone: 504-235-6328
Email: [email protected]
Website: www.SalamanderScience.com

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

Amphibians
Hellbender Salamander (Cryptobranchus alleganiensis)

With which agencies have you partnered with on eDNA applications?
Virginia Department of Game and Inland Fisheries
Western Pennsylvania Conservancy
Buffalo State University
Maryland Department of Natural Resources
The Wilds
Northeast Association of Fish and Wildlife Agencies

Amphibians, Crustacea, DNA Marker Development, Dougherty Plain cave crayfish (Cambarus cryptodytes), Flatwoods Salamander (Ambystoma bishopi and cingulatum), GA, Georgia Blind Salamander (Haideotriton wallacei), Gopher Frog (Lithobates capito), Multi Species Panel Development, Protocol Development, Research, Sample Processing, Striped Newt (Notophthalmus perstriatus)

Anna McKee

Anna McKee
Ecologist
US Geological Survey
1770 Corporate Drive Suite 500
Norcross, GA 30093
Phone: 678-924-6671
Email: [email protected]

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

Amphibians
• Flatwoods Salamanders – Frosted and Reticulated (Ambystoma cingulatum and A. bishopi)
• Georgia Blind Salamander (Haideotriton wallacei)
• Gopher Frog (Lithobates capito)
• Striped Newt (Notophthalmus perstriatus)

Crustacea
• Dougherty Plain cave crayfish (Cambarus cryptodytes)

With which agencies have you partnered with on eDNA applications?
U.S. Fish and Wildlife Service Georgia Department of Natural Resources
The Orianne Society
The San Antonio Zoo
University of Idaho University of Georgia

Amphibians, Bacteria, Batracochytrium Dendrobatidis, Boral Toad (Anaxyrus boreas boreas), Boreal Chorus Frog (Psuedaric maculata), DNA Marker Development, ecoli, Feathers, Fecal, Fungi, Hair, Multi Species Panel Development, Non-water eDNA, Northern Leopard Frog (Lithobates pipiens), Protocol Development, Research, Saliva, Sample Processing, Tiger Salamander (Ambystoma mavortium), WY, Wyoming Toad (Anaxyrus baxteri)

Melanie Murphy

Melanie Murphy
Assistant Professor
University of Wyoming
1000 E University Ave
Laramie WY 82071
Phone: 307-766-5295
Email: [email protected]
Website: https://sites.google.com/site/murphylabuwyo/

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

Amphibian
• Boral Toad (Anaxyrus boreas boreas)
• Boreal Chorus Frog (Psuedaric maculata)
• Northern Leopard Frog (Lithobates pipiens)
• Tiger salamander (Ambystoma mavortium)
• Wyoming Toad (Anaxyrus baxteri)

Bacteria
• ecoli

Fungi
• Batrachochytrium Dendrobatidis

Non-water eDNA
• Feathers
• Fecal
• Hair
• Saliva

With which agencies have you partnered with on eDNA applications?
USFS (pilot study), WDGF (permitted, sharing data – not a formal contract at this point)

Amphibians, Bog turtle (Glyptemys muhlenbergii), Burmese python (Python molurus bivittatus), DNA Marker Development, Multi Species Panel Development, NC, Protocol Development, Reptiles, Research, Sample Processing

Michael E. Dorcas

Michael E. Dorcas
Professor of Biology
Davidson College
Department of Biology
Davidson NC 28035-7118
Phone: 704-894-2727
Email: [email protected]
Website: sites.davidson.edu/dorcas

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research

Reptiles
• Bog Turtles (Glyptemys muhlenbergii)
• Burmese Python (Python molurus bivittatus)

Amphibians

With which agencies have you partnered with on eDNA applications?
North Carolina Wildlife Resource Commission

Amphibians, Bog turtle (Glyptemys muhlenbergii), DNA Marker Development, Flatwoods Salamander (Ambystoma bishopi and cingulatum), Gopher Frog (Lithobates capito), Hellbender Salamander (Cryptobranchus alleganiensis), Mudpuppy (Necturus maculosus), Multi Species Panel Development, Patch-nosed Salamander (Urspelerpes brucei), Protocol Development, Reptiles, Research, Sample Processing, Striped Newt (Notophthalmus perstriatus)

Stephen Spear

Dr. Stephen Spear
Associate Conservation Scientist
The Orianne Society
100 Phoenix Road
Athens, GA 30605
Phone: 706-353-7800
Email: [email protected]
Website: www.oriannesociety.org

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

Amphibian
• Flatwoods Salamander (Ambystoma bishopi and cingulatum)
• Gopher Frog (Lithobates capito)
• Hellbender (Cryptobranchus alleganiensis)
• Mudpuppy (Necturus maculosus)
• Patchnosed salamander (Urspelerpes brucei)
• Striped Newt (Notophthalmus perstriatus)

Reptiles
• Bog turtle (Glyptemys muhlenbergii)

With which agencies have you partnered with on eDNA applications?
United States Geological Survey, North Carolina Wildlife Resources Commission, Georgia Department of Natural Resources, Tennessee Wildlife Resources Agency

Black Carp (Mylopharyngodon piceus), Boa constrictor (Boa constrictor), Burmese python (Python molurus bivittatus), Catfish (Loricariidae Family), DNA Marker Development, Fish, FL, Grass Carp (Ctenopharyngodon idella), Green anaconda (Eunectes murinus), Mammal, Multi Species Panel Development, Northern African rock python (Python sebae), Protocol Development, Reptiles, Research, Sample Processing, West Indian manatee (Trichechus manatus), Yellow anaconda (Eunectes notaeus)

Margaret Hunter

Margaret Hunter
Research Geneticist
US Geological Survey
7920 NW 71st St
Gainesville, FL 32653
Phone: 352-264-3484
Email: [email protected]
Email: http://fl.biology.usgs.gov/genetics/index.html

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

Fish
• Black Carp (Mylopharyngodon piceus)
• Catfish (Loricariidae Family)
• Grass Carp (Ctenopharyngodon idella)

Mammal
• West Indian manatee (Trichechus manatus)

Reptiles
• Burmese Pythons (Python molurus bivittatus or Python bivittatus)
• Boa Constrictor (Boa constrictor)
• Green Anaconda (Eunectes murinus)
• Northern African rock python (Python sebae)
• Yellow Anaconda (Eunectes notaeus)

With which agencies have you partnered with on eDNA applications?
US Fish and Wildlife Service, National Park Service, Florida Fish and Wildlife Conservation Commission, USGS

Amphibians, Birds, Black Banded Sunfish (Enneacanthus chaetodon), Brook Trout (Salvelinus fontinalis), Brown Trout (Salmo trutta), Bull Trout (Salvelinus confluentus), Caddisfly (Order Trichoptera), Chinook Salmon (Oncorhynchus tshawytscha), Crustacea, DNA Marker Development, Fish, Idaho Giant Salamander (Dicamptodon aterrimus), Insects, Lake Trout (Salvelinus namaycush), Mammal, Mayfly (Order Ephemerotpera), MT, Multi Species Panel Development, Mysis Shrimp (Mysis mysis), Osprey (Pandion haliaetus), Protocol Development, Rainbow Trout (Oncorhynchus mykiss), Research, River Otter (Lontra canadensis), Sample Processing, Stonefly (Order Plecoptera), Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi), Yellowstone Cutthroat Trout (Oncorhynchus clarkii bouvieri)

Kellie Carim

Kellie Carim, Ph.D.
eDNA Coordinator
USDA Forest Service Rocky Mountain Research Station/ National Genomics Center for Wildlife and Fish Conservation
800 E. Beckwith Ave.
Missoula, MT 59801
Phone: 651-335-4289
Email: [email protected]

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

Amphibians
• Idaho Giant Salamanders (Dicamptodon aterrimus)

Bird
• Osprey (Pandion haliaetus)

Crustacea
• Mysis Shrimp (Order Mysida)

Fish
• Black Banded Sunfish (Enneacanthus chaetodon)
• Brook Trout (Salvelinus fontinalis)
• Brown Trout (Salmo trutta)
• Bull Trout (Salvelinus confluentus)
• Chinook Salmon (Oncorhynchus tshawytscha)
• Lake Trout (Salvelinus namaycush)
• Rainbow Trout (Oncorhynchus mykiss)
• Westslope Cutthroat Trout (Oncorhynchus clarkii lewisi)
• Yellowstone Cutthroat Trout (Oncorhynchus clarkii bouvieri)
Insects
• Caddisfly (Order Trichoptera)
• Mayfly (Order Ephemerotpera)
• Stonefly (Order Plecoptera)

Mammal
• River Otter (Lontra canadensis)

With which agencies have you partnered with on eDNA applications?
-Arizona Game and Fish Department
-California Department of Fish and Wildlife
-Idaho Fish and Game
-Montana Fish, Wildlife and Parks
-Nevada Department of Wildlife -Utah Division of Wildlife Resources -Oregon Department of Fish and Wildlife
-Nez Perce Tribe (Idaho)
-United States Forest Service (across USFS Region 1 and 3, as well as biologists at Uncompahgre and Gunnison National Forest, Boise National Forest, and Payette National Forest)

Non-agency groups:
-Colorado State University
-Trout Unlimited
-Hart Crowser Consulting

Amphibians, Chinook Salmon (Oncorhynchus tshawytscha), Coho Salmon (Oncorhynchus kisutch), Columbia Spotted Frog (Rana luteiventris), DNA Marker Development, Fish, ID, Idaho Giant Salamander (Dicamptodon aterrimus), Multi Species Panel Development, Protocol Development, Research, Rocky Mountain Tailed Frog (Ascaphus montanus), Sample Processing

David Pilliod

David Pilliod
Supervisory Research Ecologist
US Geological Survey Forest and Rangeland Ecosystem Science Center
970 Lusk Street
Boise, ID 83706
Phone: 208-426-5202
Email: [email protected]
Website: http://fresc.usgs.gov/research/researchPage.aspx?Research_Page_ID=126
►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

Amphibians
• Columbia Spotted Frog, Rana luteiventris
• Idaho Giant Salamander, Dicamptodon aterrimus
• Rocky Mountain Tailed Frog, Ascaphus montanus

Fish
• Chinook Salmon, Oncorhynchus tshawytscha
• Coho Salmon, Oncorhynchus kisutch

With which agencies have you partnered with on eDNA applications?
USFS, US FWS, Idaho Department Fish and Game

CO, DNA Marker Development, Multi Species Panel Development, Other, Protocol Development, Research, Sample Processing

John Wood

John Wood
Pisces Molecular
1600 Range Street, Ste. 201
Boulder, CO  80301
Phone: 303-546-9300
Email: [email protected]
Website: www.pisces-molecular.com

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research
►Expert Input

• many / other

With which agencies have you partnered with on eDNA applications?
BOR, USGS, USFWS, USFS, NPS, CPW, UDWR, NDWR, NMDGF, TPW

Amphibians, DNA Marker Development, Hellbender Salamander (Cryptobranchus alleganiensis), MD, Multi Species Panel Development, Protocol Development, Research, Sample Processing

Scott McDaniel

Scott McDaniel
President
Susquehannock Wildlife Society, Inc
P.O. Box 287
Bel Air, MD 21014
Phone: 443-333-9453
Email:  [email protected]
Website: www.susquehannockwildlife.org

►Multi Species Panel Development
►DNA Marker Development
►Protocol Development
►Sample Processing
►Research

• Eastern Hellbender (Cryptobranchus alleganiensis)

With which agencies have you partnered with on eDNA applications?
Smithsonian Institute, Maryland Department of Natural Resources